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Drug behandling og<em> In vivo</em> Imaging af Osteoblast-osteoklaster Interaktioner i en Medaka Fish Osteoporose Model
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Drug Treatment and In Vivo Imaging of Osteoblast-Osteoclast Interactions in a Medaka Fish Osteoporosis Model

Drug behandling og<em> In vivo</em> Imaging af Osteoblast-osteoklaster Interaktioner i en Medaka Fish Osteoporose Model

DOI:
10.3791/55025-v

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08:53 min

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January 01, 2017

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,
1Department of Biological Sciences,National University of Singapore, 2NUS Centre for Bioimaging Sciences (CBIS),National University of Singapore

Chapters

  • 00:05Title
  • 01:05Embryo Collection and Transgenic Embryo Screening
  • 03:07Medaka-larvae Bisphosphonate Treatment
  • 04:24Live Medaka-larvae Imaging
  • 05:56Results: Representative Transgenic Marker Expression Analyses in 12 – 15 DPF Medaka-larvae
  • 07:24Conclusion

Summary

자동 번역

자동 번역

Small laboratory fish have become popular models for bone research on the mechanisms underlying human bone disorders and for the screening of bone-modulating drugs. In this report, we describe a protocol to assess the effect of alendronate on bone cells in medaka larvae with osteoporotic lesions.

Tags

Keywords: BoneOsteoporosisMedakaIn Vivo ImagingOsteoblastOsteoclastTransgenicDrug TestingSkeletal ResearchEmbryoHeat ShockFluorescence ImagingCathepsinEctopic Osteoclasts

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