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Chapters
- 00:05Title
- 00:55Preparation of Xenopus Oocytes
- 01:45Microinjection of mRNA into Cytoplasm
- 03:41Strip Follicles
- 05:15Results: Voltage Clamp Experiment
- 06:37Conclusion
Optimized procedures for the isolation of single follicles, cytoplasmic RNA microinjections, the removal of surrounding cell layers, and protein expression in Xenopus oocytes are described. In addition, a simple method for fast solution changes in electrophysiological experiments with ligand-gated ion channels is presented.
