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1Department of Biochemistry and Molecular Biology and Smart-aging Convergence Research Center, College of Medicine,Yeungnam University, 2Physiology and Experimental Medicine Program,The Hospital for Sick Children Research Institute, 3Department of Laboratory Medicine and Pathobiology,University of Toronto
Chapters
- 00:05Title
- 00:49Mouse Embryonic Stem Cell (mESC) Culture
- 02:06Serum- and Feeder-free Conditioned Medium Collection
- 03:17Senescence-associated (SA) Beta Galactosidase Assay
- 04:46Cell Cycle and qRT-PCR Analyses
- 07:19Results: Characterization of the Effects of mESC-conditioned Medium on Cell Growth, Cycle Stage, and Function
- 08:29Conclusion
Ce protocole fournit une méthode pour la collecte de cellules souches embryonnaires de souris (MESC) de moyenne Climatisé (MESC-CM) dérivé de sérum (sérum fœtal bovin, FBS) - et d'alimentation (fibroblastes embryonnaires de souris, MEF) conditions sans gluten pour une cellule approche -free. Il peut être applicable pour le traitement du vieillissement et de maladies associées au vieillissement.
