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Isolation and Flow Cytometric Analysis of Human Endocervical Gamma Delta T Cells
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Isolation and Flow Cytometric Analysis of Human Endocervical Gamma Delta T Cells

Isolation and Flow Cytometric Analysis of Human Endocervical Gamma Delta T Cells

DOI:
10.3791/55038-v

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08:10 min

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February 06, 2017

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, , ,
1Department of Microbiology and Immunology,Miller School of Medicine, University of Miami, 2Division of Infectious Diseases,Miller School of Medicine, University of Miami

Chapters

  • 00:05Title
  • 01:01Endocervical Cytobrush Sample Processing
  • 02:11Endocervical Cell Flow Cytometric Analysis
  • 03:34Gamma Delta T Cell Isolation
  • 05:37Results: Representative Human Endocervical Gamma Delta T Cell Analyses
  • 06:50Conclusion

Summary

자동 번역

자동 번역

We describe here an isolation method to obtain human endocervical intraepithelial lymphocytes for the analysis of intraepithelial gamma delta T cells. This protocol can be extended for the purification of endocervical gamma delta T cells by magnetic beads or by cell sorting.

Tags

EndocervicalGamma Delta T CellsFlow CytometryIsolationCharacterizationIntraepithelialImmune EventsCytobrushIMDM MediumCell StrainerFlow Cytometry AnalysisAntibodiesViability DyeCompensation ControlsMagnetic Bead IsolationTCR Gamma Delta

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