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Chapters
- 00:05Title
- 00:50Cryopreservation
- 02:04Collecting Cryosections for RNA Extraction and Histology
- 04:20RNA Isolation from Pooled Cryosections
- 06:17Results: RNA Extraction and Histology of Cardiotoxin Injured Muscle
- 07:40Conclusion
Consecutive cryo-sections are collected to enable histological applications and enrichment of RNA for gene expression measurements using adjacent regions from a single mouse skeletal muscle. High-quality RNA is obtained from 20 - 30 mg of pooled cryosections and measurements are directly compared across applications.
