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Un sistema de microfluidos con modelar superficie de Investigación de la burbuja de cavitación (s) Interacción -Cell y los Bioefectos resultante en el nivel de celda única
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A Microfluidic System with Surface Patterning for Investigating Cavitation Bubble(s)–Cell Interaction and the Resultant Bioeffects at the Single-cell Level
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Un sistema de microfluidos con modelar superficie de Investigación de la burbuja de cavitación (s) Interacción -Cell y los Bioefectos resultante en el nivel de celda única

DOI:
10.3791/55106-v

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11:14 min

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January 10, 2017

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, , , ,
1Mechanical Engineering and Materials Science,Duke University, 2Huacells Corp

Chapters

  • 00:05Title
  • 00:49Gold Dot Patterning
  • 04:19Fabricating the Molecular-assembly Patterning by Lift-off (MAPL)
  • 06:17Chip Assembly and Bubble Investigation
  • 09:11Results: Shear Stress and Membrane Dynamics of Single Cells from Bubble Interactions
  • 10:46Conclusion

Summary

자동 번역

자동 번역

A microfluidic chip was fabricated to produce pairs of gold dots for tandem bubble generation and fibronectin-coated islands for single-cell patterning nearby. The resultant flow field was characterized by particle image velocimetry and was employed to study various bioeffects, including cell membrane poration, membrane deformation, and intracellular calcium response.

Tags

Keywords: Microfluidic SystemSurface PatterningCavitation BubbleCell InteractionBioeffectsSingle-cell LevelTandem BubblesTransit CavitationMicrofabricationPhotolithographySpin CoatingGold DotNegative PhotoresistChrome MaskUV ExposureDeveloper Solution

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