The Power of Simplicity: Sea Urchin Embryos as <em>in Vivo</em> Developmental Models for Studying Complex Cell-to-cell Signaling Network Interactions

Ryan C. Range; Marina Martinez-Bartolom&#233;; Stephanie D. Burr

doi:10.3791/55113

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The Power of Simplicity: Sea Urchin Embryos as in Vivo Developmental Models for Studying Complex Cell-to-cell Signaling Network Interactions

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The Power of Simplicity: Sea Urchin Embryos as in Vivo Developmental Models for Studying Complex Cell-to-cell Signaling Network Interactions

The Power of Simplicity: Sea Urchin Embryos as in Vivo Developmental Models for Studying Complex Cell-to-cell Signaling Network Interactions

DOI:

10.3791/55113-v

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07:34 min

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February 16, 2017

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Ryan C. Range, Marina Martinez-Bartolomé, Stephanie D. Burr

¹Department of Biological Sciences,Mississippi State University

Chapters

00:05Title
00:51Microinjection of Morpholino Oligonucleotides
02:31Fixation
03:40Hybridization
04:54In Situ Development
05:40Slide Preparation and Image Capture
06:22Results: Identification of Molecules Involved in Wnt Anterior-posterior Specification
07:01Conclusion

Summary

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This video article details a straightforward in vivo methodology that can be used to systematically and efficiently characterize components of complex signaling pathways and regulatory networks in many invertebrate embryos.

The Power of Simplicity: Sea Urchin Embryos as in Vivo Developmental Models for Studying Complex Cell-to-cell Signaling Network Interactions

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