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Chapters
- 00:05Title
- 01:25MSC Expansion and Preparation of Activated MSC Spheroids in 3-D Hanging Drop Cultures
- 04:50Intraperitoneal Delivery of Intact Spheroids
- 08:02Results: Spheroids Produced Using MSC Hanging Drop Cultures
- 09:31Conclusion
The therapeutic potential of mesenchymal stem/stromal cells (MSCs) is well-documented, however the best method of preparing the cells for patients remains controversial. Herein, we communicate protocols to efficiently generate and administer therapeutic spherical aggregates or 'spheroids' of MSCs primed under xeno-free conditions for experimental and clinical applications.
