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1Center for Metabolic and Degenerative Diseases, The Brown Foundation Institute of Molecular Medicine, Programs in Human and Molecular Genetics and Neuroscience, The Graduate School of Biomedical Sciences at Houston,The University of Texas Health Science Center at Houston, 2Department of Natural Sciences,University of Houston – Downtown, 3Department of Neurobiology and Anatomy, McGovern Medical School,The University of Texas Health Science Center at Houston
Chapters
- 00:05Title
- 01:12Microsurgical Dissection of Adult Fly Heads
- 02:56Immunofluorescent Staining of the Brains
- 04:44Mounting and Imaging of Brain Tissue
- 06:02Results: Anti-TH Staining in Dissected Fly Brains Allows Quantification of DA Neurons
- 07:40Conclusion
El adulto de Drosophila cerebro es un sistema valioso para el estudio de los circuitos neuronales, las funciones cerebrales superiores, y los trastornos complejos. Un método eficaz para diseccionar el tejido cerebral entera de la cabeza pequeña mosca que facilitará los estudios basados en el cerebro. Aquí se describe un protocolo de disección simple, de un solo paso de cerebros adultos con una morfología bien conservada.
