Wild-type Blocking PCR Combined with Direct Sequencing as a Highly Sensitive Method for Detection of Low-Frequency Somatic Mutations

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DOI:

10.3791/55130-v

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10:41 min

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March 29, 2017

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Adam Z. Albitar, Wanlong Ma, Maher Albitar

¹NeoGenomics Laboratories

Chapters

00:05Title
00:43Wild-Type Blocking PCR: Oligonucleotide Design
03:54Purification of PCR Product by Magnetic Beads
05:53Purification of the Sequencing Products
08:17Identification of MYD88 Mutations in Human Samples
10:03Conclusion

Summary

자동 번역

Wild-type blocking PCR followed by direct sequencing offers a highly sensitive method of detection for low frequency somatic mutations in a variety of sample types.

Wild-type Blocking PCR Combined with Direct Sequencing as a Highly Sensitive Method for Detection of Low-Frequency Somatic Mutations

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Chapters

Summary

자동 번역

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