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在子宫内电途径研究神经元亚群的兴奋性和单细胞连接
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In Utero Electroporation Approaches to Study the Excitability of Neuronal Subpopulations and Single-cell Connectivity
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在子宫内电途径研究神经元亚群的兴奋性和单细胞连接

DOI:
10.3791/55139-v

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10:49 min

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February 15, 2017

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, , ,
1Department for Molecular and Cellular Biology,Centro Nacional de Biotecnología (CNB-CSIC), 2Molecular Neurobiology Department, Centro de Biología Molecular “Severo Ochoa”,Consejo Superior de Investigaciones Científicas (CSIC-UAM)

Chapters

  • 00:05Title
  • 00:48Injection of DNA and Electroporation
  • 01:52Sectioning and Staining of Brain Tissue
  • 04:17Imaging and Analysis
  • 05:42Electrophysiology
  • 08:29Results: Representative Firing Pattern of a CAG-GFP Electroporated Pyramidal Neuron
  • 09:39Conclusion

Summary

자동 번역

자동 번역

这份手稿规定， 在子宫内电（IUE）用来描述在单细胞水平与荧光标记的神经元兴奋性神经元的结构连接协议。组织学是用于表征树突和轴突突起。在急性切片全细胞记录被用来研究兴奋性。

Tags

In Utero ElectroporationNeuronal SubpopulationsSingle-cell ConnectivityDevelopmental NeurobiologyCognitive DisordersSparse Population Of NeuronsDendritesPatch Clamp StudyLateral VentricleCryoprotectionCryosectioningImmunohistochemistry

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