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实验室规模的生产和治疗性抗体的纯化
JoVE 신문
생화학
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JoVE 신문 생화학
Laboratory Scale Production and Purification of a Therapeutic Antibody
DOI:

09:54 min

January 24, 2017

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Chapters

  • 00:05Title
  • 00:38Stable Transfection of HEK-293 Cells
  • 03:59Large Scale Antibody Production from Batch Overgrowth Culture
  • 05:12Antibody Purification by Affinity Chromatograpy Using an Automated Fast Protein Liquid Chromatography System
  • 07:02Results: Laboratory-scale Production and Purification of Antibody
  • 08:50Conclusion

Summary

자동 번역

这个协议描述在哺乳动物表达系统中生产的治疗性抗体的。所描述的方法包括制备载体DNA,稳定转染和人胚肾293细胞系的无血清适应的,设置大规模培养和纯化使用亲和层析组成。

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