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DNA 콘텐츠 측정에 의한 포유 동물 복제 타이밍의 게놈 전체의 결정
JoVE 신문
유전학
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JoVE 신문 유전학
Genome-wide Determination of Mammalian Replication Timing by DNA Content Measurement
DOI:

08:06 min

January 19, 2017

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Chapters

  • 00:05Title
  • 00:40Collection of Cells from Culture
  • 01:34Fixation
  • 02:31Propidium Iodide Staining
  • 03:39Sorting
  • 05:32DNA Sonication
  • 06:19Results: Measurements of S/G1 DNA Content Produce Detailed Time of Replication Maps
  • 07:34Conclusion

Summary

자동 번역

We describe here a relatively fast and simple approach for mapping genome-wide mammalian replication timing, from cell isolation to the basic analysis of the sequencing results. A genomic map of a representative replication program will be provided following the protocol.

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