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एकाधिक प्रतिदीप्ति द्वारा इंटर-गुणसूत्र स्थिर aberrations की जांच<em> बगल में</em> संकरण (mFISH) और विकिरणित चूहे में स्पेक्ट्रल Karyotyping (आकाश)
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Detection of Inter-chromosomal Stable Aberrations by Multiple Fluorescence In Situ Hybridization (mFISH) and Spectral Karyotyping (SKY) in Irradiated Mice
DOI:

10:14 min

January 11, 2017

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Chapters

  • 00:05Title
  • 00:53Isolation of Bone Marrow Mononuclear Cells by Density Gradient Centrifugation and Preparation of Metaphase Cell Spreads
  • 03:04Mouse Chromosome Painting by mFISH
  • 06:07Spectral Karyotyping of Mouse Chromosomes
  • 07:45Results: Inter-chromosomal Stable Aberration Analysis by Multiple Fluorescence In Situ Hybridization and Spectral Karyotyping
  • 09:02Conclusion

Summary

자동 번역

The present protocol describes the usefulness of multiple fluorescence in situ hybridization (mFISH) and spectral karyotyping (SKY) in identifying inter-chromosomal stable aberrations in the bone marrow cells of mice after exposure to total body irradiation.

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