Journal
/
/
Rilevazione di Inter-aberrazioni cromosomiche stabili per fluorescenza multipla<em> In Situ</em> Ibridazione (mFISH) e spettrale cariotipo (SKY) in topi irradiati
JoVE 신문
유전학
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 유전학
Detection of Inter-chromosomal Stable Aberrations by Multiple Fluorescence In Situ Hybridization (mFISH) and Spectral Karyotyping (SKY) in Irradiated Mice
DOI:

10:14 min

January 11, 2017

, ,

Chapters

  • 00:05Title
  • 00:53Isolation of Bone Marrow Mononuclear Cells by Density Gradient Centrifugation and Preparation of Metaphase Cell Spreads
  • 03:04Mouse Chromosome Painting by mFISH
  • 06:07Spectral Karyotyping of Mouse Chromosomes
  • 07:45Results: Inter-chromosomal Stable Aberration Analysis by Multiple Fluorescence In Situ Hybridization and Spectral Karyotyping
  • 09:02Conclusion

Summary

자동 번역

The present protocol describes the usefulness of multiple fluorescence in situ hybridization (mFISH) and spectral karyotyping (SKY) in identifying inter-chromosomal stable aberrations in the bone marrow cells of mice after exposure to total body irradiation.

Related Videos

Read Article