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1Department of Biological Sciences,Clemson University, 2Department of Marine Biodiversity Research (BioDive),Japan Agency for Marine-Earth Science and Technology (JAMSTEC), 3Advanced Material Research Laboratory (AMRL),Clemson University, 4Swire Institute of Marine Sciences and School of Biological Sciences,The University of Hong Kong
Chapters
- 00:00Title
- 04:11Screening for Life Stage and Tissue of Interest with Live Imaging
- 06:20Sample Preservation, Dehydration and Mounting for Electron Microscopy
- 08:00Location a Calcium Rich Region Using SEM-EDS
- 09:11Identifying Crystallographic Information of Calcium Rich Regions Using SEM-EBSD
- 10:28TEM Sample Preparation Using FIB-SEM
- 12:32Obtaining Selected Area Diffraction Pattern on a TEM
- 13:47Representative Results
- 15:05Conclusion
We demonstrate the use of various microscopy methods that are useful in observing the calcification of a tubeworm, Hydroides elegans, as well as locating and characterizing the first calcified material. Live microscopy and electron microscopy are used together to provide functional and material information that are important in studying biomineralization.
