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Preparation of Horizontal Slices of Adult Mouse Retina for Electrophysiological Studies
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Preparation of Horizontal Slices of Adult Mouse Retina for Electrophysiological Studies

Preparation of Horizontal Slices of Adult Mouse Retina for Electrophysiological Studies

DOI:
10.3791/55173-v

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07:02 min

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January 27, 2017

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,
1Department of Biology,Friedrich-Alexander-University (FAU) Erlangen-Nürnberg

Chapters

  • 00:05Title
  • 00:57Dissection of Mouse Retina
  • 03:12Embedding of Retinal Pieces in Agarose
  • 04:14Mounting of Retinal Pieces
  • 04:58Results: Synaptic Activity Recorded from a Horizontal Cell Body
  • 06:24Conclusion

Summary

자동 번역

자동 번역

We developed a horizontal slice preparation of the adult mammalian retina with the plane of sectioning parallel to the retinal surface. Dendritic fields of nonradially oriented retinal neurons were not truncated, allowing the study of signal processing with patch-clamp and imaging techniques.

Tags

Keywords: Horizontal SlicesAdult Mouse RetinaElectrophysiological StudiesRetinal NeuronsRibbon SynapsesVisual Information ProcessingHorizontal CellsAmacrine CellsEyeball DissectionCornea RemovalVitreous Body RemovalRetina Isolation

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