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活细胞成像和血管紧张素的三维分析受体1a型贩运转染人胚肾细胞使用共聚焦显微镜
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JoVE 신문 생물학
Live Cell Imaging and 3D Analysis of Angiotensin Receptor Type 1a Trafficking in Transfected Human Embryonic Kidney Cells Using Confocal Microscopy
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活细胞成像和血管紧张素的三维分析受体1a型贩运转染人胚肾细胞使用共聚焦显微镜

DOI:
10.3791/55177-v

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09:51 min

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March 27, 2017

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, , , ,
1Department of Biochemistry,Georgetown University Medical Center, 2Department of Medicine,Georgetown University Medical Center, 3Department of Physics,Georgetown University Medical Center, 4Department of Oncology,Georgetown University Medical Center

Chapters

  • 00:05Title
  • 00:49Live Cell Imaging
  • 02:46Image Analysis
  • 07:08Results: Representative AT1aR-GFP Expression and Analyses
  • 08:46Conclusion

Summary

자동 번역

자동 번역

在这里，我们提出了一个协议，以表达绿色荧光蛋白标记的血管紧张素1a型内吞过程中血管紧张素Ⅱ开始治疗受体细胞图像。这种技术包括用第二荧光标记物标记的溶酶体，然后利用软件随时间来分析受体和溶酶体的共定位在三维空间中。

Tags

Live Cell Imaging3D AnalysisAngiotensin Receptor Type 1aTraffickingTransfected Human Embryonic Kidney CellsConfocal MicroscopyQuantitative Spatial And Temporal EvidenceAngiotensin Two TreatmentSubcellular ProcessingY Type ReceptorMutant ReceptorInternalization ProcessLigand StimulationNumerical Aperture ObjectiveImmersion OilZ StackXYZT ModeLigand Stimulation3D Image QuantificationGFP Tagged Receptor VesicleHole Cell IntensitiesExtended Focus ModeFreestyle Region Tool

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