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可诱导T7 RNA聚合酶介导的多基因表达系统，pMGX

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Inducible T7 RNA Polymerase-mediated Multigene Expression System, pMGX

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可诱导T7 RNA聚合酶介导的多基因表达系统，pMGX

DOI:

10.3791/55187-v

•

10:09 min

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June 27, 2017

•

Mohamed I. Hassan*¹, Fern R. McSorley*¹, Kinya Hotta, Christopher N. Boddy

¹Department of Chemistry and Biomolecular Sciences,University of Ottawa, ²School of Biosciences,The University of Nottingham Malaysia Campus

Chapters

00:05Title
00:49Cloning Genes of Interest in the Multigene Expression System Vector, pMGX
04:11Insert Gene 2 into the pMGX Vector Containing Gene 1, pMGX-yfg1
07:08Results: Multigene Expression in pMGX
09:10Conclusion

Summary

자동 번역

该研究描述了使用pMGX质粒系统在大肠杆菌中使用单个质粒的多个基因的T7介导的共表达的方法。

Tags

Keywords: Inducible T7 RNA Polymerase Multigene Expression System PMGX Synthetic Biology 생화학 Protein Complexes Co-expression Escherichia Coli Gene Cloning Restriction Enzyme Digestion Gel Electrophoresis Gel Extraction Ligation Bacterial Transformation Antibiotic Selection

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