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Een fluorescentie gebaseerde lymfocyt Assay geschikt voor high-throughput screening van kleine moleculen
JoVE 신문
면역학 및 감염병학
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JoVE 신문 면역학 및 감염병학
A Fluorescence-based Lymphocyte Assay Suitable for High-throughput Screening of Small Molecules
DOI:

08:43 min

March 10, 2017

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Chapters

  • 00:05Title
  • 00:40Generation of Splenocyte Cell Suspension from Nur77GFP Mouse Spleens
  • 02:07T Cell Isolation from the Splenocyte Cell Suspension
  • 03:26T-cell Activation and the Induction of GFP Expression
  • 04:42High Throughput Screening of Small Molecule
  • 06:05Results: T and B Cell Receptors Activation Flowcytometry Results and a Representative T Cell HTS of Smal Molecules
  • 07:56Conclusion

Summary

자동 번역

We presenteren in de huidige studie een nieuwe fluorescentie gebaseerde assay middels lymfocyten afkomstig van een transgene muis. Deze test is geschikt voor high-throughput screening (HTS) van kleine moleculen begiftigd met de hoedanigheid van het remmen of bevorderen van lymfocyt activering.

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