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表面上のヒトノロウイルスの検出のためのスワブサンプリング方法
JoVE 신문
면역학 및 감염병학
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JoVE 신문 면역학 및 감염병학
Swab Sampling Method for the Detection of Human Norovirus on Surfaces
DOI:

10:03 min

February 06, 2017

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Chapters

  • 00:05Title
  • 01:04Swab Sampling, Virus Concentration, Viral RNA Extraction, and Purification
  • 02:36Midi Column Viral Nucleic Acid Extraction
  • 03:40Viral Nucleic Acid Concentration
  • 05:16Multiplex RT-qPCR Detection
  • 07:09Quantification of Norovirus in Swab Samples and Hemi-nested Conventional PCR Amplification
  • 08:11Results: Macrofoam-based Swab Sampling Method Effectively Detects Norovirus Outbreaks
  • 09:24Conclusion

Summary

자동 번역

A macrofoam based sampling methodology was developed and evaluated for the detection and quantification of norovirus on environmental hard surfaces.

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