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Funktionel Manipulation af Maternal Gene produkter ved hjælp af<em> In vitro</em> Oocytmodning i Zebrafisk
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Functional Manipulation of Maternal Gene Products Using In Vitro Oocyte Maturation in Zebrafish

Funktionel Manipulation af Maternal Gene produkter ved hjælp af<em> In vitro</em> Oocytmodning i Zebrafisk

DOI:
10.3791/55213-v

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10:39 min

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April 22, 2017

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, , , ,
1Laboratory of Genetics,University of Wisconsin-Madison, 2Department of Biological Sciences,Tata Institute of Fundamental Research, 3Department of Genetics, Cell Biology, and Development,University of Minnesota

Chapters

  • 00:05Title
  • 00:50Pre-selection of Females and Preparation of Maturation Medium
  • 02:29Dissection of Oocytes and Initiation of In Vitro Culture
  • 04:56Oocyte Microinjection
  • 05:52Oocyte Maturation and Defolliculation
  • 07:18Fertilization of In Vitro-cultured Oocytes
  • 08:15Results: Maternal Gene Product Manipulation Using In Vitro Oocyte Maturation
  • 09:36Conclusion

Summary

자동 번역

자동 번역

En optimeret protokol til in vitro modning af zebrafisk oocytter anvendes til manipulation af maternelle genprodukter præsenteres her.

Tags

In Vitro Oocyte MaturationZebrafishMaternal Gene Products발생학FertilizationViable EmbryosMating TankLeibovitz’s L-15 MediumDHPGentamicinTricaineOocyte DissectionLight Cycle

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