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系统抑制剂靶向细胞内的疗效和毒性筛选<em>结核分枝杆菌</em
JoVE 신문
면역학 및 감염병학
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JoVE 신문 면역학 및 감염병학
System for Efficacy and Cytotoxicity Screening of Inhibitors Targeting Intracellular Mycobacterium tuberculosis
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系统抑制剂靶向细胞内的疗效和毒性筛选<em>结核分枝杆菌</em

DOI:
10.3791/55273-v

•

09:57 min

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April 05, 2017

•

,
1Department of Medicine,University of British Columbia

Chapters

  • 00:05Title
  • 00:58THP-1 Medium and Maintenance and High-throughput Intracellular Screening Using Luciferase-expressing M. tuberculosis H37Rv
  • 03:54Cytotoxicity Analysis Using an MTT Assay
  • 06:06In-broth Activity Analysis Using a Resazurin Assay
  • 07:16Results: Efficacy Screening of Inhibitors of M. tuberculosis
  • 08:53Conclusion

Summary

자동 번역

자동 번역

我们已经开发了模块化高通量筛选系统，用于发现新化合物对结核分枝杆菌 ，靶向细胞内和细胞在肉汤中生长的细菌以及细胞毒性对哺乳动物宿主细胞。

Tags

Mycobacterium TuberculosisIntracellular InfectionMacrophagesLuciferase AssayCytotoxicityMICDrug DevelopmentTHP1 CellsOpsonizationMOIDifferentiation96-well PlateCompound Screening

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