Journal
/
생체공학
/
Polydimethylsiloxane-polykarbonat microfluidic Enheter for celle migrasjon Studies Under Perpendicular Kjemisk og Oksygen Graderinger
JoVE 신문
생체공학
This content is Free Access.
JoVE 신문 생체공학
Polydimethylsiloxane-polycarbonate Microfluidic Devices for Cell Migration Studies Under Perpendicular Chemical and Oxygen Gradients

Polydimethylsiloxane-polykarbonat microfluidic Enheter for celle migrasjon Studies Under Perpendicular Kjemisk og Oksygen Graderinger

DOI:
10.3791/55292-v

•

11:23 min

•

February 23, 2017

•

, , , ,
1Research Center for Applied Sciences,Academia Sinica

Chapters

  • 00:05Title
  • 01:20Fabrication of the PC-embedded Top Layer
  • 02:59Assembly of the Device
  • 04:42Microfluidic Cell Migration Assay
  • 08:38Results: Characterization of Chemical and Oxygen Gradients Established within the Microfluidic Device, and Study of Cell Migration Under Gradient Combinations
  • 10:35Conclusion

Summary

자동 번역

자동 번역

The control of chemical and oxygen gradients is essential for cell cultures. This paper reports a polydimethylsiloxane-polycarbonate (PDMS-PC) microfluidic device capable of reliably generating combinations of chemical and oxygen gradients for cell migration studies, which can be practically utilized in biological labs without sophisticated instrumentation.

Tags

Keywords: Polydimethylsiloxane-polycarbonateMicrofluidic DeviceCell MigrationChemical GradientOxygen GradientPDMSPolycarbonateMammalian CellsCell MotilityCell-based Drug TestingCancer Cell Metastasis

Article

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

check_url/kr/55292?article_type=v

Enkle Microfluidic enheter for<em> In vivo</em> Imaging av<em> C. elegans</em>,<em> Drosophila</em> Og Sebrafisk
check_url/kr/55292?article_type=v

Opprette Lim og Løselig overgangar for Imaging Cell Migration med fluorescens mikroskopi
check_url/kr/55292?article_type=v

Microfabrication av nanoporøse Gold Patterns for Cell-materielle Interaksjonsstudier
check_url/kr/55292?article_type=v

Millifluidics for kjemisk syntese og Time-løst mekanistiske studier
check_url/kr/55292?article_type=v

Cell klemme som en robust, Microfluidic Intracellulær Levering Platform
check_url/kr/55292?article_type=v

Lag-på-lag Kollagen Nedfall i mikrofluid Enheter for Microtissue Stabilization
check_url/kr/55292?article_type=v

Ved hjelp av lim Mønstring å konstruere 3D Paper microfluidic enheter
check_url/kr/55292?article_type=v

Microfluidic Buffer Exchange for Forstyrrelsesfri Micro / partikler Cell Engineering
check_url/kr/55292?article_type=v

Designing mikrofluid Enheter for å studere cellulære responser Under Singel eller andre sykdoms Kjemisk / Elektro / Shear Stress Stimuli
check_url/kr/55292?article_type=v

Multi-trinns variabel Høyde Fotolitografi for Valved Multilayer microfluidic enheter

Read Article