Journal
/
/
Solea Senegalensis精子中活性氧的细胞内定位评价
JoVE 신문
발생학
Author Produced
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 발생학
Evaluation of Intracellular Location of Reactive Oxygen Species in Solea Senegalensis Spermatozoa
DOI:

10:24 min

March 11, 2018

,

Chapters

  • 00:00Title
  • 01:32Preparatory work before the experiment
  • 02:58Sample preparation before fluorochromes incubation
  • 04:40Fluorochromes incubation
  • 05:33Sample preparation for confocal microscopy
  • 05:51Confocal setting up prior to the experiment
  • 06:49Acquisition of images
  • 07:49Results
  • 08:24Creating a 3D image video
  • 09:53Conclusions

Summary

자동 번역

此协议描述了一个用于检测Solea senegalensis精子中的 H2O2本地化的详细方法, 使用敏感的 fluorochrome DCFH-DA 为 ROS、线粒体的活线粒体染色和细胞核的 DAPI可视化, 分别。该协议的设计是在2小时内进行, 无论是新鲜或解冻精子。

Related Videos

Read Article