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1School of Biological Sciences,University of Hong Kong, 2Department of Mechanical Engineering,University of Hong Kong, 3Department of Medicine,University of Hong Kong, 4Department of Biomedical Sciences, Key Laboratory of Biochip Technology, Biotech and Health Centre,Shenzhen Research Institutes of City University of Hong Kong
Chapters
- 00:05Title
- 00:49Microfluidic Device Master Design
- 02:32Polydimethylsiloxane Chip Fabrication
- 04:26Seeding the Microfluidic Chip with Primary Peritoneal Mesothelial Cells
- 06:08Ovarian Cancer Spheroid Formation and Co-culture
- 08:09Perfusion Platform Assembly and Co-culture
- 09:16Results: Ovarian Cancer Spheroid Behavior in a 3D Peritoneal Microfluidic Device
- 10:09Conclusion
To study ovarian tumor progression in a physiologically relevant model, multicellular spheroids were cultured in a microdevice under simulated fluid flow. This dynamic 3D model emulates the intraperitoneal environment with the cellular and mechanical components where ovarian cancer metastasis occurs.
