/
/
Dinamik 3D Periton microdevice Modelleme Yumurtalık Kanseri Multisellüler Sfero Davranış
A subscription to JoVE is required to view this content. Sign in or start your free trial.
1School of Biological Sciences,University of Hong Kong, 2Department of Mechanical Engineering,University of Hong Kong, 3Department of Medicine,University of Hong Kong, 4Department of Biomedical Sciences, Key Laboratory of Biochip Technology, Biotech and Health Centre,Shenzhen Research Institutes of City University of Hong Kong
Chapters
- 00:05Title
- 00:49Microfluidic Device Master Design
- 02:32Polydimethylsiloxane Chip Fabrication
- 04:26Seeding the Microfluidic Chip with Primary Peritoneal Mesothelial Cells
- 06:08Ovarian Cancer Spheroid Formation and Co-culture
- 08:09Perfusion Platform Assembly and Co-culture
- 09:16Results: Ovarian Cancer Spheroid Behavior in a 3D Peritoneal Microfluidic Device
- 10:09Conclusion
fizyolojik olarak ilgili model yumurtalık tümör progresyonunu incelemek için, çok hücreli küreler simüle sıvı akışı altında microdevice kültürlenmiştir. Bu dinamik 3D modeli yumurtalık kanseri metastazı meydana hücresel ve mekanik bileşenleri ile intraperitoneal ortamı benzetilmiştir.
