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The Ex Vivo Colon Organ Culture and Its Use in Antimicrobial Host Defense Studies
JoVE 신문
면역학 및 감염병학
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JoVE 신문 면역학 및 감염병학
The Ex Vivo Colon Organ Culture and Its Use in Antimicrobial Host Defense Studies
DOI:

10:40 min

February 13, 2017

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Chapters

  • 00:05Title
  • 00:49Collection and Preparation of the Colon
  • 02:30Colon Organ Culture
  • 04:43E. coli Killing Assay
  • 06:26Effect of Extrinsic and Intrinsic Factors on Colonic Antimicrobial Host Defense Responses
  • 08:51Results: Intestinal Antimicrobial Immune Responses in Ex Vivo Cultured Colon
  • 10:11Conclusion

Summary

자동 번역

The ex vivo organ culture allows investigation of biological processes in the context of the intact tissue architecture. Here, we introduce a method of ex vivo culture of the mouse colon, which can be used to study innate immunity and antimicrobial host defense in the intestine.

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