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Streamlined Single Cell TCR Isolation and Generation of Retroviral Vectors for In Vitro and In Vivo Expression of Human TCRs
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
1Department of Pediatrics, Section of Diabetes and Endocrinology, McNair Medical Institute,Baylor College of Medicine, Texas Children’s Hospital, 2Benaroya Research Institute at Virginia Mason, 3Center for Human Immunobiology,Baylor College of Medicine, Texas Children’s Hospital, 4Department of Pediatrics, Section of Diabetes and Endocrinology,Baylor College of Medicine, Texas Children’s Hospital
Chapters
- 00:00Title
- 01:42Single cell sort and reverse transcription reaction
- 03:37Multiplex-nested PCR
- 08:56Subcloning TCR alpha and beta chains
- 09:56Verification of TCR expression
- 10:46Conclusion
