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En protokoll for å karakterisere de morfologiske endringene av<em> Clostridium difficile</em> Som svar på antibiotisk behandling
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
Chapters
- 00:05Title
- 01:54Isolate C. difficile from Different Environmental or Clinical Sources
- 03:46Culturing C. difficile and Killing Kinetic Procedures
- 04:53Prepare Samples for Scanning Electron Microscopy
- 06:26Imaging C. difficile Cells on a Scanning Electron Microscope
- 09:21Image Processing and Analysis
- 10:08Results: Morphological Analysis of C. difficile in Response to Antibiotic Treatment
- 11:40Conclusion
Antibiotic efficacy is most commonly determined by conducting killing kinetic studies and measuring colony forming units (CFUs). By integrating scanning electron microscopy (SEM) with these standard methods, we can distinguish the pharmacological effects of treatment between different antibiotics.
