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Expression of Exogenous Cytokine in Patient-derived Xenografts via Injection with a Cytokine-transduced Stromal Cell Line
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Expression of Exogenous Cytokine in Patient-derived Xenografts via Injection with a Cytokine-transduced Stromal Cell Line

Expression of Exogenous Cytokine in Patient-derived Xenografts via Injection with a Cytokine-transduced Stromal Cell Line

DOI:
10.3791/55384-v

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12:58 min

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May 10, 2017

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, , , , , , ,
1Department of Pathology and Human Anatomy,Loma Linda University, 2Department of Basic Sciences,Loma Linda University, 3Department of Medicine,Loma Linda University

Chapters

  • 00:05Title
  • 01:22Culture and Expansion of Cytokine Transduced Stromal Cells
  • 03:05Phospho-flow Cytometry Analysis of Stroma-produced Cytokine Activity
  • 05:09Injection of Cytokine-transduced Stroma into Mice
  • 06:22Serial Blood Collection and Plasma Monitoring of Exogenous Human Cytokine in Mice
  • 08:33Human Hematopoietic Cell Transplantation
  • 09:53Results: Achieving Physiological Levels of the Human TSLP Cytokine in PDX Mice
  • 11:22Conclusion

Summary

자동 번역

자동 번역

Described here is a method for producing exogenous cytokine in patient-derived xenograft (PDX) mice via weekly intraperitoneal injection of a cytokine-transduced stromal cell line. This method broadens the utility of PDX and provides the option for transient or sustained exogenous cytokine delivery in a multitude of PDX models.

Tags

Keywords: CytokineXenograftPatient-derivedStromal Cell LineHematopoiesisLeukemiaTSLPCell CultureELISACell StorageCell Lines

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