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Chapters
- 00:05Title
- 00:58Preparation of Agarose-coated Plates
- 03:06Cell Culture and Multicellular Tumor Spheroid Formation
- 06:17Medium Exchange Using a Robotic System
- 07:14High-content Imaging of MCTS and Semi-automated Image Analysis
- 08:04Analysis of Multicellular Spheroid Formation in the Presence of Anti-cancer Drugs
- 10:16Conclusion
The uneven loss of medium from microtiter plates affects the reproducibility of uniform multicellular tumor spheroid formation. Improving culture conditions to reduce significant medium loss will improve the reproducibility of spheroid formation and the results of spheroid-based assays using the liquid-overlay technique.
