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Peptid-Scanning-gestützte Identifizierung eines monoklonalen Antikörpers anerkannte Linear B-Zell-Epitop
JoVE 신문
면역학 및 감염병학
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JoVE 신문 면역학 및 감염병학
Peptide Scanning-assisted Identification of a Monoclonal Antibody-recognized Linear B-cell Epitope
DOI:

08:09 min

March 24, 2017

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Chapters

  • 00:05Title
  • 00:55Preparation of Monoclonal Antibody
  • 02:24Construction and Expression of Serially Truncated Recombinant Proteins
  • 03:45Dot-blot Hybridization
  • 06:24Results: Recognition of the Epitope Region by Dot-blot Hybridization
  • 07:44Conclusion

Summary

자동 번역

Here, the authors present a simple and efficient protocol to define a linear antigenic epitope using a purified monoclonal antibody and peptide scanning through dot-blot hybridization. The identified epitope can then be used in therapeutic and diagnostic applications.

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