Journal
/
생물학
/
A Rapid Automated Protocol for Muscle Fiber Population Analysis in Rat Muscle Cross Sections Using Myosin Heavy Chain Immunohistochemistry
JoVE 신문
생물학
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 생물학
A Rapid Automated Protocol for Muscle Fiber Population Analysis in Rat Muscle Cross Sections Using Myosin Heavy Chain Immunohistochemistry

A Rapid Automated Protocol for Muscle Fiber Population Analysis in Rat Muscle Cross Sections Using Myosin Heavy Chain Immunohistochemistry

DOI:
10.3791/55441-v

•

05:57 min

•

March 28, 2017

•

, , , , , ,
1CD Laboratory for the Restoration of Extremity Function, Division of Plastic and Reconstructive Surgery, Department of Surgery,Medical University of Vienna, 2Core Facility Imaging, Core Facilities,Medical University Vienna, 3Department of Hand, Plastic and Reconstructive Surgery, Burn Center, BG Trauma Center Ludwigshafen, Plastic and Hand Surgery,University of Heidelberg

Chapters

  • 00:05Title
  • 00:43Staining
  • 02:16Microscopy
  • 03:45Muscle Fiber Quantification
  • 04:43Results: Representative Muscle Fiber Analyses of a Rat Muscle Cross Section
  • 05:27Conclusion

Summary

자동 번역

자동 번역

Here, we present a protocol for rapid muscle fiber analyses, which allows improved staining quality, and thereby automatic acquisition and quantification of fiber populations using the freely available software ImageJ.

Tags

Muscle Fiber AnalysisImmunohistochemistryMyosin Heavy ChainRat MuscleAutomated ProtocolImage AcquisitionDAPINeuromuscularAgingDiseaseTraumaSkeletal MuscleCross-sectionQuantificationOpen-sourceSlide ScannerImage Processing

Article

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

Procedures for Rat in situ Skeletal Muscle Contractile Properties

Fluorescence-based Measurement of Store-operated Calcium Entry in Live Cells: from Cultured Cancer Cell to Skeletal Muscle Fiber

Analysis of Skeletal Muscle Defects in Larval Zebrafish by Birefringence and Touch-evoke Escape Response Assays

Generation of Myospheres From hESCs by Epigenetic Reprogramming

Optical Cross-Sectional Muscle Area Determination of Drosophila Melanogaster Adult Indirect Flight Muscles

Sequential Immunofluorescence and Immunohistochemistry on Cryosectioned Zebrafish Embryos

Preparation of Rat Skeletal Muscle Homogenates for Nitrate and Nitrite Measurements

A Simple and Inexpensive Running Wheel Model for Progressive Resistance Training in Mice

Author Spotlight: Mitochondrial Remodeling in Skeletal Muscle

Author Spotlight: Decellularization-Based Quantification of Skeletal Muscle Fatty Infiltration

Read Article