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JoVE Journal Neuroscience
Evaluación de la Viabilidad Neuronal Utilizando Colorante de Fluoresceína-Ioduro de Propidio Doble Coloración en Gránulos Cerebelosos Cultura de Neuronas
 
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Evaluación de la Viabilidad Neuronal Utilizando Colorante de Fluoresceína-Ioduro de Propidio Doble Coloración en Gránulos Cerebelosos Cultura de Neuronas

Article DOI: 10.3791/55442-v • 08:53 min • May 10th, 2017
May 10th, 2017 •
*1, *2, 1, 1, 1, 2, 3, 1, 2,4, 1,2
1Ningbo Key Laboratory of Behavioral Neuroscience, Zhejiang Provincial Key Laboratory of Pathophysiology, School of Medicine, Ningbo University, 2Department of Applied Biology and Chemistry Technology, Institute of Modern Chinese Medicine, The Hong Kong Polytechnic University, 3Institute of New Drug Research, Guangdong Provincial Key Laboratory of Pharmacodynamic, Constituents of Traditional Chinese Medicine & New Drug Research, College of Pharmacy, Jinan University, 4International Joint Laboratory (SYSU-PolyU HK) of Novel Anti-Dementia Drugs of Guangdong
* These authors contributed equally

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Este protocolo describe cómo medir con precisión la viabilidad neuronal utilizando fluoresceína diacetato (FDA) y Propidium Iodide (PI) doble tinción en neuronas cultivadas de gránulos cerebelosos, un cultivo neuronal primario utilizado como un modelo in vitro en neurociencia y neurofarmacología investigación.

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Neurociencia Número 123 neuronas de gránulos cerebelosos diacetato de fluoresceína yoduro de propidio viabilidad celular células gliales microscopía de fluorescencia
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