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Invasive Behavior of Human Breast Cancer Cells in Embryonic Zebrafish
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Invasive Behavior of Human Breast Cancer Cells in Embryonic Zebrafish

Invasive Behavior of Human Breast Cancer Cells in Embryonic Zebrafish

DOI:
10.3791/55459-v

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09:22 min

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April 25, 2017

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, , ,
1Department of Molecular Cell Biology, Cancer Genomics Centre Netherlands,Leiden University Medical Center

Chapters

  • 00:05Title
  • 01:28Preparation of Fluorescent Cells
  • 02:42Preparation of Zebrafish Embryos
  • 03:20Xenotransplantation into the Perivitelline Space
  • 05:07Xenotransplantation into the Duct of Cuvier
  • 05:47Invasion and Metastasis Assessment
  • 06:32Results: Xenotransplanted MDA-MB-231, MCF-10A, and MCF-10A-Ras Cells Reveal Distinct Invasiveness Patterns
  • 08:17Conclusion

Summary

자동 번역

자동 번역

Here, we describe xenograft zebrafish models using two different injection sites, i.e., perivitelline space and duct of Cuvier, to investigate the invasive behavior and to assess the intravasation and extravasation potential of human breast cancer cells, respectively.

Tags

Invasive BehaviorHuman Breast Cancer CellsEmbryonic ZebrafishMetastasisIntravasationExtravasationAnti-metastatic ActivityPeritumoral AngiogenesisCancer Cell DormancyMDA-MB-231 CellsMCF10A CellsMCF10A-Ras CellsCell ImplantationMicroinjection

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