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Chapters
- 00:05Title
- 01:12Preparation of the Microfluidic Device
- 05:17Time-resolved ElectroSpray Ionization Hydrogen-deuterium Exchange
- 06:51Results: Detection of Protein Structural Changes by Time-resolved ElectroSpray Ionization Hydrogen-deuterium Mass Spectrometry
- 08:10Conclusion
Konformationel fleksibilitet spiller en afgørende rolle i protein funktion. Heri, beskriver vi anvendelsen af tidsopløst elektrospray massespektrometri koblet til hydrogen-deuterium gengæld for probing de hurtige strukturændringer, som driver funktion i ordnede og uordnede proteiner.
Tags
Time-resolved Electrospray IonizationHydrogen-deuterium ExchangeMass SpectrometryProtein StructureProtein DynamicsConformational FlexibilityTransient Protein ConformationsLoop RegionsMolten GlobulesIntrinsically Disordered ProteinsNeurodegenerative DisordersProtein MisfoldingProtein AggregationEnzyme Catalytic TurnoverProtein-protein InteractionsProtein-ligand InteractionsContinuous FlowTime-resolved Kinetic MixerOrthogonal Mixing
