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Gebruik makend van pHluorin-gelabeld receptoren Monitor subcellulaire lokalisatie en handel
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JoVE 신문 생물학
Utilizing pHluorin-tagged Receptors to Monitor Subcellular Localization and Trafficking
DOI:

09:59 min

March 16, 2017

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Chapters

  • 00:05Title
  • 00:39Live Cell Imaging by TIRFM
  • 02:48Imaging SEP to Determine Subcellular Localization
  • 04:39Imaging Single Vesicle Insertion Events in Live Cells
  • 05:44Image Analysis and Data Processing
  • 08:17Results: Monitoring the Localization, Expression, and Trafficking of Membrane Proteins
  • 09:30Conclusion

Summary

자동 번역

Labelen van de extracellulaire domein van een membraaneiwit met een pH-gevoelige fluorofoor, superecliptic pHluorin (SEP), maakt lokalisatie, expressie en mensenhandel worden bepaald. Imaging september-gemerkte eiwitten met totale interne reflectie fluorescentie microscopie (TIRFM) maakt de kwantificering van eiwitniveaus in de perifere ER en plasmamembraan.

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