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在嵌合斑马鱼胚胎的个别视网膜祖细胞转基因表达的体内成像来研究细胞非自治的影响
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In Vivo Imaging of Transgenic Gene Expression in Individual Retinal Progenitors in Chimeric Zebrafish Embryos to Study Cell Nonautonomous Influences
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在嵌合斑马鱼胚胎的个别视网膜祖细胞转基因表达的体内成像来研究细胞非自治的影响

DOI:
10.3791/55490-v

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10:36 min

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March 22, 2017

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, , ,
1School of Biosciences,The University of Melbourne, 2Australian Regenerative Medicine Institute (ARMI),Monash University, 3The David J Apple Center for Vision Research, Department of Ophthalmology,Heidelberg University

Chapters

  • 00:05Title
  • 00:52Embryo Microinjection
  • 02:53Preparation for Transplantation
  • 04:59Chimera Generation via Blastula Transplantation
  • 06:37Mounting for Live Imaging
  • 08:27Results: Transgene Expression in Retinal Progenitors from Donor Cells
  • 09:34Conclusion

Summary

자동 번역

자동 번역

在不同的遗传背景的个体WT视网膜祖细胞的现场跟踪允许细胞非自主性的信令神经过程中贡献进行评估。这里，通过胚胎移植和体内的时间推移共焦成像的基因敲除，嵌合体的产生的组合物用于此目的。

Tags

In Vivo ImagingTransgenic Gene ExpressionRetinal Progenitor CellsChimeric Zebrafish EmbryosCell Non-autonomous InfluencesFate SpecificationMicroinjectionH2AGFPH2BRFPMRNAFluorescence Microscopy

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