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斑马鱼<em>原位</em>脊髓制备用于脊髓感觉和运动神经元的电生理记录
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Zebrafish In Situ Spinal Cord Preparation for Electrophysiological Recordings from Spinal Sensory and Motor Neurons
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斑马鱼<em>原位</em>脊髓制备用于脊髓感觉和运动神经元的电生理记录

DOI:
10.3791/55507-v

•

08:24 min

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April 18, 2017

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, ,
1Department of Physiology and Biophysics,University of Colorado Anschutz Medical Campus (UCAMC), 2Neuroscience Graduate Program,University of Colorado Anschutz Medical Campus (UCAMC)

Chapters

  • 00:05Title
  • 00:48Preparation of Disseciton Materials
  • 02:32Dissection of Embryo for Patch-clamp Recordings from Spinal Neurons
  • 07:00Results: Excitability Properties of Bohon-Beard Neurons
  • 07:51Conclusion

Summary

자동 번역

자동 번역

这手稿描述了从斑马鱼胚胎和幼虫的脊髓神经元的电生理记录的方法。制备维持神经元在原地 ，往往涉及到最小的清扫。这些方法允许对多种脊髓神经元的电生理学研究中，从通过早期幼体阶段的初始电兴奋采集。

Tags

ZebrafishIn SituSpinal CordElectrophysiologySpinal NeuronsSensory NeuronsMotor NeuronsEmbryoLarvaRohon Beard NeuronsSilicone ElastomerDissection ChamberRinger’s SolutionTricaineMicropipette

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