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면역학 및 감염병학

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体外和活体评估移植受体的 T、B 和髓细胞抑制活性和体液反应

JoVE 신문
면역학 및 감염병학
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JoVE 신문 면역학 및 감염병학

In Vitro and In Vivo Assessment of T, B and Myeloid Cells Suppressive Activity and Humoral Responses from Transplant Recipients

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体外和活体评估移植受体的 T、B 和髓细胞抑制活性和体液反应

DOI:

10.3791/55510-v

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18:48 min

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August 12, 2017

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Séverine Bézie², Claire Usal², Carole Guillonneau²

¹Centre de Recherche en Transplantation et Immunologie UMR 1064,INSERM, Université de Nantes, ²Institut de Transplantation Urologie Néphrologie (ITUN),CHU Nantes

Chapters

00:00Title
00:37LEW.1W to LEW.1A Allograft Procedure
02:18Isolation of Allograft APCs (pDCs)
06:12Isolation of Responder Effector T Cells and Regulatory T Cells (CD4⁺ and CD8⁺CD45RC^low)
10:30CFSE Labeling of Responder Cells to Measure their Proliferation
12:09FACS Staining and Analysis After 6 Days of Coculture
13:49In Vivo Assessment of the Cells Supressive Activity by Adoptive Cell Transfer in a Heart Grafted Recipient
16:33Representative Results
18:03Conclusion

Summary

자동 번역

在这里, 我们提出了一个诱导耐受性的移植, 并评估体外和体内的抑制能力的不同细胞亚群从接受者和免疫状态的受体向施主或外源性抗原。

Tags

Keywords: Transplant Tolerance Regulatory Cells Suppressive Capacity Humoral Response In Vitro In Vivo Splenocytes Lewis Rat Collagenase EDTA Cell Isolation

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