Journal
/
발생학
/
Induction of Mesenchymal-Epithelial Transitions in Sarcoma Cells
JoVE 신문
발생학
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 발생학
Induction of Mesenchymal-Epithelial Transitions in Sarcoma Cells

Induction of Mesenchymal-Epithelial Transitions in Sarcoma Cells

DOI:
10.3791/55520-v

•

11:42 min

•

April 07, 2017

•

, , , , , , , , , , , ,
1Department of Medicine,Duke University, 2Department of Bioengineering,Rice University, 3Department of Molecular Genetics and Microbiology,Duke University, 4Solid Tumor Program and the Duke Prostate Center,Duke University Medical Center, 5Duke University Medical Center

Chapters

  • 00:05Title
  • 01:01Lentiviral Transduction of GRHL2
  • 06:00Sorting for EGFP+ Cells by Flow Cytometry
  • 07:14Reverse Transfection of miR-200s
  • 09:03Results: Representative Analysis of Tranduction Induced MET
  • 11:10Conclusion

Summary

자동 번역

자동 번역

We present here a cell culture method for inducing mesenchymal-epithelial transitions (MET) in sarcoma cells based on combined ectopic expression of microRNA-200 family members and grainyhead-like 2 (GRHL2). This method is suitable for better understanding the biological impact of phenotypic plasticity on cancer aggressiveness and treatments.

Tags

Mesenchymal-epithelial TransitionSarcomaPhenotypic PlasticityGene Regulatory NetworksTherapeutic InterventionsHEK293T CellsTransfectionLentivirus Production

Article

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

Direct Induction of Human Neural Stem Cells from Peripheral Blood Hematopoietic Progenitor Cells

Direct Induction of Hemogenic Endothelium and Blood by Overexpression of Transcription Factors in Human Pluripotent Stem Cells

Chondrogenic Differentiation Induction of Adipose-derived Stem Cells by Centrifugal Gravity

Directed Differentiation of Primitive and Definitive Hematopoietic Progenitors from Human Pluripotent Stem Cells

Induction of Hypoxia in Living Frog and Zebrafish Embryos

Horizontal Whole Mount: A Novel Processing and Imaging Protocol for Thick, Three-dimensional Tissue Cross-sections of Skin

Single-cell Photoconversion in Living Intact Zebrafish

Computational Analysis of the Caenorhabditis elegans Germline to Study the Distribution of Nuclei, Proteins, and the Cytoskeleton

Induction and Validation of Cellular Senescence in Primary Human Cells

Induction of Endothelial Differentiation in Cardiac Progenitor Cells Under Low Serum Conditions

Read Article