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Protocols for Investigating the Host-tissue Distribution, Transmission-mode, and Effect on the Host Fitness of a Densovirus in the Cotton Bollworm
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Chapters
- 00:05Title
- 00:40Construction of the HaDV2-free Cotton Bollworm Colony
- 02:02Preparation of HaDV2-containing Liquid Fluids
- 03:28Construction of the HaDV2-infected Cotton Bollworm Colony
- 04:57Tissue Distribution of HaDV2
- 07:01Bioassays Testing the Effect of HaDV2 on Host Development
- 09:36Results: Characterization of Effects of Densovirus on Bollworms
- 10:44Conclusion
Here, we present a protocol to investigate the host-tissue distribution, transmission mode, and effect on the host fitness of a densovirus within a lepidopteran species, the cotton bollworm. This protocol can also be used for studying the interaction between other orally-transmitted viruses and their insect hosts.
