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Chapters
- 00:05Title
- 00:48Preoperative Procedures
- 01:45DRG Injection and Dorsal Root Crush Injury
- 05:08Postoperative Procedures
- 06:34Anterograde CTB Injection for Axonal Tracing
- 07:25Results: Sensory Axon Regeneration Visualized with GFP and CTB after Dorsal Root Crush Injury
- 08:59Conclusion
This protocol presents the use of a dorsal root ganglion (DRG) injection with a viral vector and a concurrent dorsal root crush injury in an adult rat as a model to study sensory axon regeneration. This model is suitable for investigating the use of gene therapy to promote sensory axon regeneration.
Tags
Keywords:
Dorsal Root GanglionDorsal Root Crush InjurySensory Axon RegenerationSpinal CordAxon RegenerationCNS Axon RegenerationSurgical ApproachNeuronal Cell BodyTherapeutic InterventionAnimal WelfareSurgical ProcedureAnesthesiaAnalgesiaAntibioticStereotaxic FrameMuscle LayersSpinous ProcessesDissection
