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激光局部补骨脂素加合物的单分子分析

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Single Molecule Analysis of Laser Localized Psoralen Adducts

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激光局部补骨脂素加合物的单分子分析

DOI:

10.3791/55541-v

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11:46 min

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April 20, 2017

•

Jing Huang, Himabindu Gali, Julia Gichimu, Marina A. Bellani, Durga Pokharel, Manikandan Paramasivam, Michael M. Seidman

¹Laboratory of Molecular Gerontology,National Institute on Aging, National Institutes of Health

Chapters

00:05Title
00:53Calibrating the Targeting Laser
04:54Laser Localized Dig-TMP ICLs
07:44Harvesting Cells and Stretching DNA Fibers
09:51Results: Laser Localized Psoralen Adducts Reveal the DNA Damage Response to ICLs
11:21Conclusion

Summary

자동 번역

激光器在对DNA损伤的细胞应答的研究经常使用的。然而，它们产生的病变，其间隔，频率，和碰撞与复制叉很少表征。在这里，我们描述了一种方法，使这些参数与激光局部间交联的决心。

Tags

Keywords: Single Molecule Analysis Laser Localized Psoralen Adducts DNA Interstrand Crosslinks DNA Repair Laser Activated Compound Mirror Slide Calibration Laser Targeting Biological Calibration Trimethylpsoralen GFP Tagged Repair Factor U2OS Cell Line

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