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Use of a Central Venous Line for Fluids, Drugs and Nutrient Administration in a Mouse Model of Critical Illness
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Use of a Central Venous Line for Fluids, Drugs and Nutrient Administration in a Mouse Model of Critical Illness

Use of a Central Venous Line for Fluids, Drugs and Nutrient Administration in a Mouse Model of Critical Illness

DOI:
10.3791/55553-v

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09:17 min

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May 02, 2017

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, , , , ,
1Clinical Division and Laboratory of Intensive Care Medicine, Department of Cellular and Molecular Medicine,KU Leuven

Chapters

  • 00:05Title
  • 00:54Venous Line Preparation and Chronic Indwelling Catheter Placement
  • 05:06Cecal Ligation and Puncture (CLP)
  • 07:06Results: Representative Survival Curves after 5 or 7 Days of Critical Illness
  • 08:08Conclusion

Summary

자동 번역

자동 번역

This protocol describes a centrally catheterized mouse model of prolonged critical illness. We combine the cecal ligation and puncture method to induce sepsis with the use of a central venous line for fluids, drugs and nutrient administration to mimic the human clinical setting.

Tags

Keywords: Central Venous LineFluidsDrugsNutrient AdministrationMouse ModelCritical IllnessCecal Ligation And Puncture (CLP)Intensive CareEndocrineMetabolicAnesthesiaSurgical TechniqueCatheterWire LoopMouse PositioningSkin IncisionMuscle Exposure

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