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1The Solomon H. Snyder Department of Neuroscience,Johns Hopkins University School of Medicine, 2Department of Neurology and neurosurgery,Johns Hopkins University School of Medicine, 3Departments of Pharmacology and Molecular Sciences,Johns Hopkins University School of Medicine, 4Department of Psychiatry and Behavioral Sciences,Johns Hopkins University School of Medicine
Chapters
- 00:05Title
- 00:37Expression of Recombinant HA-MOR1, Cell Fractionation, and Membrane Collection
- 03:55[35S]GTPγS Binding
- 06:05Membrane Filtration
- 06:59Liquid Scintillation Counting
- 07:57Results: Characterization of G-protein-coupled Receptors by Monitoring Radio-labled GTP Binding
- 09:01Conclusion
Guanosintriphosphat (GTP) -Bindung ist eine der frühesten Ereignisse in der G-Protein-Coupled Receptor (GPCR) -Aktivierung. Dieses Protokoll beschreibt die pharmakologisch charakterisierende spezifische GPCR-Ligand-Wechselwirkungen durch die Überwachung der Bindung des radioaktiv markierten GTP-Analogs [ 35 S] Guanosin-5'-O- (3-thio) triphosphats ([ 35 S] GTPγS) in Antwort auf einen Liganden von Interesse.
