JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
1The Solomon H. Snyder Department of Neuroscience,Johns Hopkins University School of Medicine, 2Department of Neurology and neurosurgery,Johns Hopkins University School of Medicine, 3Departments of Pharmacology and Molecular Sciences,Johns Hopkins University School of Medicine, 4Department of Psychiatry and Behavioral Sciences,Johns Hopkins University School of Medicine
Chapters
- 00:05Title
- 00:37Expression of Recombinant HA-MOR1, Cell Fractionation, and Membrane Collection
- 03:55[35S]GTPγS Binding
- 06:05Membrane Filtration
- 06:59Liquid Scintillation Counting
- 07:57Results: Characterization of G-protein-coupled Receptors by Monitoring Radio-labled GTP Binding
- 09:01Conclusion
Guanosintrifosfat (GTP) -bindning är en av de tidigaste händelserna vid aktivering av G-proteinkopplad receptor (GPCR). Detta protokoll beskriver hur man farmakologiskt karakteriserar specifika GPCR-ligand-interaktioner genom att övervaka bindningen av den radiomärkta GTP-analogen, [35S] guanosin-5'-0- (3-tio) trifosfat ([35S] GTPyS), i Svar på en ligand av intresse.
