JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
1Guangzhou Medical University, 2Shenzhen Key Laboratory of Tissue Engineering, Shenzhen Laboratory of Digital Orthopeadic Engineering, Department of Orthopedics,Shenzhen Second People’s Hospital (The First Hospital Affiliated to Shenzhen University), 3Department of Chemistry,The Chinese University of Hong Kong, 4School of Biomedical Engineering,Shanghai Jiao Tong University, 5Renji Hospital Clinical Stem Cell Research Center,Shanghai Jiao Tong University School of Medicine, 6Shantou University Medical College
Chapters
- 00:05Title
- 01:52Human Articular Cartilage Tissue Collection and Chondrocyte Culture
- 03:33Genomic DNA Extraction
- 05:47Dot Blot nalysis
- 07:55Results: DNA Methylation Increases During Chondrocyte Dedifferentiation
- 08:45Conclusion
Presentiamo un metodo per quantificare la metilazione del DNA in base alla macchia dot-5-metilcitosina (5-mc). Abbiamo determinato i livelli di 5 mC durante la dedifferentiation dei condrociti. Questa semplice tecnica potrebbe essere utilizzata per determinare rapidamente il fenotipo condrocitico nel trattamento ACI.
