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制备和<em>体外</em>磁化的表征的miR-改性内皮细胞
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Preparation and In Vitro Characterization of Magnetized miR-modified Endothelial Cells
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制备和<em>体外</em>磁化的表征的miR-改性内皮细胞

DOI:
10.3791/55567-v

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09:58 min

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May 02, 2017

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, , , , , ,
1Reference and Translation Center for Cardiac Stem Cell Therapy (RTC), Department of Cardiac Surgery,University of Rostock, 2Physikalisch-Technische Bundesanstalt, 3Department of Radiology and Neuroradiology,Ernst-Moritz-Arndt-University Greifswald, 4Electron Microscopy Center,University of Rostock

Chapters

  • 00:05Title
  • 00:46Transfection
  • 02:38Testing of Transfection Efficiency
  • 04:21Cell Targeting Evaluation and Magnetic Cell Separation
  • 07:06Results: Safe and Efficient Delivery of miRNA to Endothelial Cells and Their Magnetic Responsiveness
  • 08:45Conclusion

Summary

자동 번역

자동 번역

这个手稿描述的miR的有效的，非病毒递送通过PEI / MNP矢量和它们的磁化与内皮细胞。因此，除了遗传修饰，该方法允许用于磁性细胞的指导和MRI可检测性。该技术可以被用来改善的治疗性细胞产品的特性。

Tags

Magnetized CellsMiR-modified CellsEndothelial CellsCell MagnetizationMicroRNA TransfectionPolyethyleneimeneSuper Paramagnetic Iron Oxide NanoparticlesCell TherapyRegenerative MedicineCell RetentionCell SurvivalConfocal MicroscopyStructured Illumination Microscopy

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